"QUICK AND EASY" genomic prep for PCR analysis

To quickly and easily screen one or several strains of Chlamydomonas the following protocol works amazingly well:

1) resuspend a pellet of approximately 5-10 uL in size in 50 uL of 10mM NaEDTA by vortexing.

2) incubate at 100C for 5 minutes (thermocycler works well).

3) vortex to mix.

4) centifuge at 12000g for 1 minute.

5) use 1.5 uL in a 25 uL PCR reaction.

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