Plasmids in the Chlamydomonas Resource Center collection

   Unless otherwise specified, all plasmids can be assumed to be selectable on ampicillin.  
 
plasmid genome description reference more info
P-14  cp  chloroplast DNA BamH1 14/15 (3.0 kb) in pBR313
  contains 3' end of 23S rRNA gene through 5S
  see P-124 for same insert in pBR322		   web page
P-17  cp  chloroplast DNA BamHI 10 (7.6 kb) in pBR313
  contains entire atpB gene and 3' portion of ORF1995		   web page
P-19  cp  chloroplast DNA BamHI 8 (9.6 kb) in pBR313
   contains most of rpoC2 (3') and 5' end of psaB
   see P-122 for same insert in pBR322		   web page
P-21  cp  chloroplast DNA BamHI 6 (13.4 kb) in pBR313
  contains 3' end of rps2 rpoA, psbB, psbN, and psbH
  P-21 is the same insert in pBR313 		  web page
P-50  cp  chloroplast DNA BamHI 4 (20 kb) in pUC8
  contains psbZ, psbM, rps14, rps7, atpE, ycf12, and psbA
  see P-156 for same insert in pUC18		   web page
P-52  cp  chloroplast DNA BamHI 5 (16.5 kb) in pUC8
  contains psbK, tufA, rpl20, clpP, petB, chlL, rpl36, rpl23,
  rpl2, rps19, and rpl16		   web page
P-57  cp  chloroplast DNA BamHI 9 (8.4 kb) in pUC8
  contains rpl14, rps8, psaA exon 1, and rps4		   web page
P-59  cp  chloroplast DNA BamHI 11/12 (7.0 kb) in pUC8
  contains 16S rRNA through 23S 5' end		   web page
P-62  cp  chloroplast DNA EcoRI 5 (10.5 kb) in pUC8
  contains psbB, rpoA, rps2 and most of rps18 		  web page
P-63  cp  chloroplast DNA EcoRI 8 (8.5 kb) in pUC8
  contains rps8, psaA exon 1, rps4 and 5' end of 16S rRNA		   web page
P-64  cp  chloroplast DNA EcoRI 9 (7.6 kb) in pUC8
  contains clpP, rpl35, rpl23, and 5' half of rpl2		   web page
P-65  cp  chloroplast DNA EcoRI 12 (6.5 kb) in pUC8
  contains psaA exon 3		   web page
P-66  cp  chloroplast DNA EcoRI 13 (6.0 kb) in pUC8
   contains psbA exons 1-4 		  web page
P-67  cp  chloroplast DNA EcoRI 14 (5.8 kb) in pUC8
  contains psaB, rbcL, and 5' end of atpA		   web page
P-68  cp  chloroplast DNA EcoRI 15 (5.8 kb) in pUC8
  contains psbA exons 1-4		   web page
P-69  cp  chloroplast DNA EcoRI 16 (5.0 kb) in pUC8
  contains 3' end of rpl2, rps19, rpl16, rpl14, and rpl5		   web page
P-70  cp  chloroplast DNA EcoRI 17 (4.9 kb) in pUC8
  contains rps12, psaJ, atpI, and psbJ 		  web page
P-71  cp  chloroplast DNA EcoRI 18 (4.9 kb) in pUC8
  contains rps11, tscA, and most of chlN		   web page
P-72  cp  chloroplast DNA EcoRI 19 (4.4 kb) in pUC8
  contains psbH, psbN, and psbT		   web page
P-73  cp  chloroplast DNA EcoRI 24 (3.3 kb) in pUC8
  contains ycf12 and atpE 		  web page
P-74  cp  chloroplast DNA EcoRI 26 (3.0 kb) in pUC8
  internal to ORF2971		   web page
P-75  cp  chloroplast DNA EcoRI 31 (1.4 kb) in pUC8
  internal to rpoC2		   web page
P-76  cp  chloroplast DNA EcoRI 32 (1.3 kb) in pUC8
  contains 3' half oftufA		   web page
P-78  cp  chloroplast DNA PstI 4 (15.1 kb) in pBR322
  contains rps2, rps18, ycf3, ycf4, rps9, psbE, rpoB and psbF
  tetracycline-resistant		   web page
P-82  cp  chloroplast DNA PstI 14 (3.8 kb) in pBR322
  contains psbT and psbB
  tetracycline resistant		   web page
P-84  cp  chloroplast DNA PstI 19 (2.9 kb) in pBR322
  contains rpoC1b
  tetracycline resistant		   web page
P-85  mito  mitochondrial Bam-Sal fragment (5.6 kb) in pBR322
  contains cox1, nd2, nd6, W, L6, L7, Q, and S1		   web page
P-86  cp  chloroplast DNA EcoRI 22 (3.5 kb) in pUC8
  contains nearly all of atpA, psbI, and most of cemA 		  web page
P-87  cp  chloroplast DNA BamHI 7 (11.6 kb) in pUC8
  contains part of the Wendy transposon, petA, petD, and chlB 		  web page
P-92  nuc  nuclear rDNA unit, 3.6 kb BamH1 fragment in pUC8
  contains part of 25S, 5.8S, and most of 18S rDNA		 Marco and Rochaix (1980)
  Mol. Gen. Genet. 177, 715-723		 web page
P-99  cp  chloroplast DNA EcoRI 27 (2.7 kb) in pCR1
  contains most of psbD and the 5' end of ORF2971
  kanamycin resistant		   web page
P-102  cp  chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8
  contains wild-type 16S rRNA gene (3' end)		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-104  cp  chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8
  contains 16S rRNA gene (3' end) from spectinomycin
  resistant mutant spr-u-1-6-2		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-106  cp  chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8
  contains 16S rRNA gene (3' end) from kanamycin
  resistant mutant kr-u-24-2		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-112  cp  BamHI-EcoRI (5.3 kb) in pUC8
  overlap of Bam 10 and Eco 11; contains atpB 		   
P-119  cp  700 bp RsaI fragment in pUC8
  from Kpn-Eco region of Bam 10		    
P-122  cp  chloroplast DNA BamHI 8 (9.6 kb) in pBR322
   contains most of rpoC2 (3') and 5' end of psaB
   see P-19 for same insert in pBR313		  
P-123  cp  chloroplast DNA BamHI 13 (6.8 kb) in pBR322
   contains most of ccsA and psaA exon 3		    
P-124  cp  chloroplast DNA BamHI 14/15 (3.0 kb) in pBR322
  contains 3' end of 23S rRNA gene through 5S
  see P-14 for same insert in pBR313		    
P-125  cp  chloroplast DNA BamHI 16 (1.1 kb) in pBR322
  internal to psaB		    
P-130  cp  EcoRI-KpnI (3.0 kb) in pUC18
  contains atpB		    
P-135  cp  EcoRI-KpnI (3.0 kb) in pUC18
  atpB with ac-u-c-2-29 mutation (K154N)		 Robertson et al. (1989)
  J. Biol. Chem. 264, 2331-2337		  
P-144  cp  900 bp HindIII fragment in pUC9
  internal to rbcL 		   
P-149  nuc  nuclear DNA, 0.8 kb EcoRI fragment in pUC18
  CS2.1 probe for RBCS2		 Goldschmidt-Clermont and Rahire (1986)
  J. Mol. Biol. 191, 421-432		  
P-151  nuc  3.1 kb XhoI-SmaI fragment in pUC9
  probe for alpha-1 tubulin 		   
P-152  nuc  3.1 kb XhoI fragment in pBR322
  beta-2 tubulin + 3' flank 		   
P-156  cp  chloroplast DNA BamHI 4 (20 kb) in pUC18
  contains psbZ, psbM, rps14, rps7, atpE, ycf12, and psbA
  see P-50 for same insert in pUC8		    
P-157  cp  BamHI-BglII 10 kb fragment in pUC18
   sublone of Bam 4; psbA from DCMU4 mutant		    
P-160  mito  mitochondrial BamHI-SalI fragment (0.3 kb) in pBR322
  from C. smithii; = Matagne pULG-S2		    
P-161  cp  chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8
  contains 16S rRNA gene (3' end) from
  neamine/kanamycin resistant mutant nr-u-2-1		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-166  cp  chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8
  contains 16S rRNA gene (3' end) from streptomycin
   resistant mutant sr-u-sm5		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-169  nuc  1.9 kb EcoRI fragment
  gamma subunit of CF1 chloroplast ATP synthase		 Yu et al. (1988)
  Proc. Natl. Acad. Sci. USA 85, 1369-1373 		 
P-173  cp  chloroplast DNA EcoRI 14 (5.8 kb)
  rbcL containing Spreitzer 68-4PP ts mutation (L290F)		    
P-175  cp  1.47 kb HincII-KpnI fragment in pUC118
  subclone of Eco 17; contains rps12		 Liu et al. (1989)
  J. Biol. Chem. 264, 16100-16108		  
P-177  cp  BamHI-EcoRI (4.1 kb) in pUC18
  5' end of 23S rRNA gene (wild type)
  overlap of Eco 3/4 and Bam 11/12		    
P-179  cp  BamHI-EcoRI (4.1 kb) in pUC18
  5' end of 23S rRNA gene from
  erythromycin resistant mutant er-u-37		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-183  cp  chloroplast DNA BamHI 11/12 (7.0 kb) in pUC18
  16S through 5' end of 23S rRNA gene
  contains sr-u-2-60, spr-u-1-6-2, and er-u-37 mutations		    
P-190  nuc  EcoRI-KpnI (8.0 kb) in pUC19
  genomic fragment from FUD44 mutant in the
   PSBO gene encoding the OEE1 protein		 Mayfield and Kindle (1990)
   Proc. Natl. Acad. Sci. USA 87, 2087-2091 		 
P-192  cp  BamHI-HindIII (1.6 kb) in pUC119
  3' end of 23S rRNA gene from
  erythromycin resistant mutant er-u-1a		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-198  cp  EcoRI-BamHI (2.0 kb) in pUC8
  5' end of 16S rRNA gene
  contains streptomycin resistance mutation sr-u-2-60 		Harris et al. (1989)
   Genetics 123, 281-292		  
P-199  cp  EcoRI-BamHI (2.0 kb) in pUC8
  5' end of 16S rRNA gene
  contains streptomycin resistance mutation sr-u-sm3		 Harris et al. (1989)
   Genetics 123, 281-292		  
P-200  cp  chloroplast DNA BamHI 11/12 (7.0 kb) in pUC18
  contains 16S and 5' end of 23S rRNA genes
   with sr-u-2-60, spr-u-1-6-2 and er-u-37 mutations		    
P-202  cp  chloroplast DNA BamHI 6 (13.5 kb) in pBR322
  contains 3' end of rps2 rpoA, psbB, psbN, and psbH
  P-21 is the same insert in pBR313		    
P-206  cp  PstI-XhoI (8.75 kb) in pUC18
  contains atpE and 3' end of rps7		    
P-207  cp  BamHI-XhoI (5.0 kb) in pUC118
  contains psbZ, psbM, rps14, and 5' end of rps7		    
P-210  cp  BamHI-EcoRI (7.3 kb) in pUC8
  contains aadA inserted downstream of atpB		    
P-213  cp  chloroplast DNA EcoRI 17 (4.9 kb) in pUC8
  contains rps12 with sr-u-sm2 mutation conferring
  streptomycin resistance 		Liu et al. (1989)
   J. Biol. Chem. 264, 16100-16108		  
P-214  cp  HincII-KpnI (1.47 kb) in pUC119, subclone of EcoRI 17
  contains wild-type rps12
   P-175 is the same insert in pUC118 		Liu et al. (1989)
   J. Biol. Chem. 264, 16100-16108		  
P-216  cp  HincII-KpnI (1.47 kb) in pUC 18, subclone of EcoRI 17
  contains rps12 in which Arg85 has been mutated
  to stop codon		 Liu et al. (1989)
   J. Biol. Chem. 264, 16100-16108		  
P-217  cp  EcoRI-KpnI (944 bp) in pUC119
  contains 5' end of atpE gene		    
P-228  cp  Bam-HindIII (4.8 kb) in pUC18
  16S through 5' part of 23S rRNA gene;
  contains spectinomycin resistance mutation spr-u-1-6-2
  see P-667 for the same insert in an XL1 blue strain		   pdf file
P-229  cp  BamHI-HindIII (5.9 kb) in pUC18, from C. smithii
  contains 16S through 5' end of 23S, with
  spectinomycin resistance mutation near 3' end of 16S rRNA		 Newman et al. (1990)
   Genetics 126, 875-888		  
P-230  cp  0.28 kb AatII fragment in pUC18, from BamHI 11/12
  spacer region between 16S and 23S rRNA genes;
   used as a probe for short dispersed repeat sequences
   in the chloroplast genome		    
P-235  nuc  cDNA clone, 1.9 kb NcoI-BamHI fragment in pBSKS
  containing nucleotides 619-2257 of the arylsulfatase gene 		de Hostos et al. (1989)
   Mol. Gen. Genet. 218, 229-239		  
P-236  cp  chloroplast DNA PstI 17/18 (3.0 kb) in pUC8
  contains psbA exon 4 and part of exon 3		    
P-239  cp  KpnI-HindIII (3.3 kb) in pUC18
  atpB disrupted with YEp24		 Newman et al. (1991)
   Mol. Gen. Genet. 230, 65-74 		 
P-240  cp  KpnI (3.0 kb) in pUC18
  16S rRNA gene from C. smithii spectinomycin
   resistant mutant		 Newman et al. (1990)
   Genetics 126, 875-888
   		 
P-242  cp  KpnI (1.4 kb) in pUC18
  fragment from the spacer between psbA and 5S;
  C. smithii		    
P-259  cp  chloroplast DNA XhoI 8 (11.0 kb) in BSKS+
  contains psbA, 5S, and 3' end of 23S with er-u-1a
   mutation		    
P-260  cp  KpnI (3.1 kb) in BSKS+
  contains psbA exons 4 and 5 from DCMU4 mutant		    
P-261  cp  EcoRI (5.51 kb) in pUC8
  contains 3' end of psbA, 5S, and 3' exon of 23S with
   er-u-1a mutation		    
P-262  cp  EcoRI (4.0 kb) in pUC8
  contains 3' end of psbA, 5S, and 3' exon of 23S
  with er-u-1a mutation;
  1.5 kb deletion in spacer between psbA and 5S		    
P-263  cp  BamHI-HindIII (4.8 kb) in pUC18
  16S rRNA gene from strain containing the spr-u-1-H-4 and
  nr-u-2-1 mutations		    
P-268  cp  KpnI (2.4 kb) in pUC18
  16S rRNA gene from spectinomycin resistant mutant
  spr-u-1-6-2		    
P-269  cp  ClaI (3.2 kb) in BSKS+
  psbA exons 1 and 2		    
P-270  cp  BamHI-EcoRI (4.48 kb) in pUC18 with Pst site deleted
  rbcL gene disrupted with YEp24		 Newman et al. (1991)
   Mol. Gen. Genet. 230, 65-74 		 
P-271  cp  chloroplast DNA BamHI 11/12 (7.0 kb) in pBR322
  16S through 5' end of 23S
  same insert as P-59		    
P-279  cp  chloroplast DNA EcoRI 25 (3.1 kb) in pUC18
  this fragment is internal to ORF2971		    
P-280  cp  chloroplast DNA EcoRI 20 (3.9 kb) in pUC18
  contains the 3' end of ORF2971 and the 5' end of psbC 		   
P-283  cp  HincII (1.5 kb) in pUC18
  contains the 5' end of psbD 		   
P-289  cp  HincII-SstI (0.8 kb) in pUC18
  3' flank of the psbA gene		    
P-291  cp  XhoI-SmaI (2.8 kb) in BSKS+
  5' exon of the 23S rRNA gene		    
P-292  cp  EcoRI-BamHI (4.5 kb) in BSSK-
  psbA cDNA (no introns)		    
P-296  cp  chloroplast DNA XhoI 8 (9.5 kb) in BSKS+
  psbA through 3' exon of 23S
  ca. 1.5 kb Kpn fragment was deleted between psbA and 5S;
  contains er-u-1a erythromycin resistance mutation in 23S		    
P-297  cp  Kpn I (3.1 kb) in BSKS+
  psbA exons 4 and 5 with R238K mutation in exon 4 and
  DCMU4 (S264K) in exon 5		    
P-300  cp  Kpn I (3.1 kb) in BSKS+
  psbA exons 4 and 5 with R238K mutation in exon 4		    
P-318  mito  mitochondrial DNA EcoRI-HindIII (1.5 kb) in pUC12
  contains nd4 and cob genes		    
P-319  cp  KpnI (2.4 kb) in pUC18
16S rRNA gene with spr-u-1-H-4 and nr-u-2-1 mutations		    
P-322  cp  EcoRI-XhoI (5.7 kb) in BSKS+
psbA exon 5 with DCMU4 mutation,
  5S, and 23S 3' end with er-u-1a mutation		   pdf file
P-327  cp  BamHI-EcoRI (2.5 kb) in BSKS+
contains Eco 33 + part of Eco 28		    
P-361  cp  EcoRI-(XhoI) (3.6 kb) in pUC18
psbA exon 5 with DCMU4 mutation, >5S and 23S 3' end
  Kpn deletion between psbA and 5S		    
P-377  cp  KpnI-BamHI (2.6 kb) in pUC18
5S and 3' end of 23S with er-u-1a mutation		    
P-386  cp  chloroplast DNA BamHI 11/12 in a pUC18 deletion plasmid
  contains 16S and 5' end of 23S,
  spr-u-1-27-3 mutation in 23S rRNA		    
P-387
  [pMN24]		  nuc  NIT1 genomic
  Sal I/Bgl II (14.5 kb) in pUC119		 Fernandez et al. (1989)
   Proc. Natl. Acad. Sci. USA 86, 6449-6453		 pdf file
P-388  cp  KpnI-PstI (0.9 kb) in KS+
  psbA exon 5, mutated to stop codon at aa 345		 Lers et al. (1992)
   J. Biol. Chem. 267, 17494-17497		  
P-389
   [pArg7]		  nuc  ARG7 genomic, 7.1 kb BamHI-SalI in pBR329
  can rescue arg2 and some other arg7 mutants		 Debuchy et al. (1989)
   EMBO J. 8, 2803-2809		  
P-423  cp   cp  DNA expression vector
  aadA flanked by Chlamy 5' atpA and 3' rbcL
  EcoRI-BamHI (1.9 kb) in pUC18; also see P-484		 Goldschmidt-Clermont (1991)
   Nucl. Acids Res. 19, 4083-4089		  
P-437  cp  chloroplast DNA BamHI 10 (7.6 kb) in KS+
  contains atpB gene
  same insert as P-17 but in KS+ vector		    
P-451  cp  chloroplast DNA EcoRI 30 (1.5 kb) in KS-
contains ycf3 and most of ycf4		    
P-452  nuc  cabII-1 genomic, KpnI-EcoRI (7.8 kb) in p1B176 Imbault et al. (1988)
   Gene 73, 397-407		  
P-453  nuc  cabII-1 genomic, pSP-CAB
  2.4 kb EcoRI-HindIII fragment in pSP65		 Imbault et al. (1988)
   Gene 73, 397-407
Nikaido et al. (1994)
   Plant Mol. Biol. 26, 275-284		  
P-454  nuc  3' UTR of cabII-1, 0.3 kb in pSP65
  digestion with HindIII allows synthesis
  of an antisense RNA		 Imbault et al. (1988)
   Gene 73, 397-407
Shepherd et al. (1983)
   Cell 32, 99-107		  
P-455  cp  chloroplast DNA PstI 15 (3.1 kb), vector uncertain
  contains rbcL (all but extreme 3' end), atpA (5' half)		    
P-456  nuc  alpha-tubulin with RbcS2 constitutive promoter    
P-463  cp  chloroplast aadA cassette, KpnI-SacI (4.8 kb) in BSKS-
  tscA, 5' rbcL, aadA, 3' rbcL		 Goldschmidt-Clermont (1991)
   Nucl. Acids Res. 19, 4083-4089		  
P-464  cp  chloroplast aadA cassette, KpnI-SacI (5 kb) in BSKS-
  tscA, 5' atpA, aadA, 3' rbcL		 Goldschmidt-Clermont (1991)
   Nucl. Acids Res. 19, 4083-4089		  
P-469  cp  GUS cassette, in KS+
  contains the entire atpB 5' UTR and promoter		    
P-477   recA from E. coli with internal deletion,
  in BSKS+
  flanked by atpA 5', rbcL 3'		    
P-478   recA from E. coli with internal deletion,
  in BSKS+
  flanked by atpA 5', rbcL 3'		    
P-484  cp   cp  DNA expression vector
  aadA flanked by Chlamy 5' atpA and 3' rbcL
  EcoRI-BamHI (1.9 kb)
  same insert as P-423, but in BSKS+		 Goldschmidt-Clermont (1991)
   Nucl. Acids Res. 19, 4083-4089		  
P-487  nuc  Hsp70C genomic, = pCB470 from Christoph Beck
  4 kb SalI-BamHI fragment in BSKS+ 		von Gromoff et al. (1989)
   Mol. Cell. Biol. 9, 3911-3918		  
P-505  cp  chloroplast DNA EcoRI 34 (ca. 1.1 kb) in pUC9
  contains psaA exon 2 and 3' end of psbD 		   
P-520  cp  chloroplast DNA PstI 11 (7.8 kb) in BSKS+
  contains atpA (3' end), atpH, rps11, rpoA		    
P-521  cp  chloroplast DNA PstI 13 (4.0 kb) in BSKS+
  internal to ORF2971		    
P-537  nuc  ATPC cDNA (CF1 ATP synthase, gamma subunit)
  in pTZ18R		 Yu et al. (1988)
   Proc. Natl. Acad. Sci. USA 85, 1369-1373		  
P-538  nuc  cytochrome c6 cDNA
  710 bp EcoRI fragment in pGEM1		 Merchant and Bogorad (1987)
  J. Biol. Chem. 262, 9062-9067		  
P-539  nuc  plastocyanin cDNA
  577 bp EcoRI fragment in pTZ18R		 Merchant et al. (1990)
   J. Biol. Chem. 265, 12372-12379		  
P-540  nuc  cytochrome c6 genomic
  ca. 5 kb SstI fragment in pTZ19R		 Hill et al. (1991)
   J. Biol. Chem. 266, 15060-15067		  
P-541  nuc  plastocyanin genomic
  ca. 6.5 kb KpnI fragment in pTZ19R		 Quinn et al. (1993)
   J. Biol. Chem. 268, 7832-7841		  
P-542  cp  E. coli recA with an N-end deletion
  flanked by atpA 5' and rbcL 3'
  1.97 kb in BSKS+		    
P-546  cp  0.62 kb expression cassette in BSSK+
  with 5' and 3' ends of psbA, NcoI/XbaI sites for
  insertion of any coding sequence in frame		   sequence [Word file with images}
P-547  cp  aadA flanked by psbA 5' and 3' regions
  ca. 1.5 kb in BSKS+		    
P-548  cp  aadA flanked by psbA 5' and atpB 3'
  3.6 kb in BSKS+		    
P-578  cp  chloroplast DNA EcoRI 6 (10 kb) in pBR328
  contains psbC 3', psaC, and rpoC1b
  P-583 is the same insert in a different vector		    
P-579  cp  chloroplast DNA EcoRI 13 (6.0 kb) in pBR328
  contains psbA exons 1-4 and extreme 3' end of chlN		    
P-581  cp  thought to be chloroplast DNA Eco RI fragment 23
  3.4 kb in pBR328
  could be Eco 24 instead		    
P-582  cp  chloroplast DNA EcoRI 28 (2.0 kb) in pCRI
  internal to ORF1995 		   
P-583  cp  chloroplast DNA EcoRI 6 (10 kb) in pCRI
  contains psbC 3', psaC, and rpoC1b
  P-578 is the same insert in pBR328		    
P-584  cp  chloroplast DNA EcoRI 7 (8.7 kb) in pCRI
  contains ccsA, psbZ, psbM, rps14, rps7,
  and the 5' end of atpE		    
P-585  cp  BglII-SalI (4.3 kb) in pUC18
  contains 3' end of rps3 and 5' end of rpoC2		 Fong and Surzycki (1992)
   Curr. Genet. 21, 485-497		  
P-586  cp  PstI-BglII (3.1 kb) in pUC8
  contains psbL, petG, and 5' portion of rps3
  subclone of chloroplast DNA fragment BglII 8		 Fong and Surzycki (1992)
   Curr. Genet. 21, 485-497		  
P-593  nuc  Gulliver K (left end)
  4.1 kb HindIII in pUC13
  used as a probe for the Gulliver transposon		 Ferris (1989)
   Genetics 122, 363-377		  
P-594  nuc  Gulliver N (right end)
  EcoRI/HindIII 0.6 kb in pUC13
  contains ca. 130 bp of non-Gulliver DNA,
  = probe C for MT- locus		 Ferris (1989)
   Genetics 122, 363-377		  
P-597  cp  chloroplast DNA PstI 22 (2.3 kb) in BSKS+
  intergenic region between rpoC1a and rpoC1b		    
P-603  cp  chloroplast DNA PstI 12 (7.5 kb) in BSKS+
  contains tscA, chlN, and psbA exons 1 and 2		    
P-606  cp  chloroplast DNA BamHI 17 (800 bp) in pTZ18R
  contains 3' end and UTR of ccsA		    
P-611  nuc  OEE2 cDNA, 1.4 kb EcoRI fragment in pUC19 Mayfield et al. (1987)
  Proc. Natl. Acad. Sci. USA 84, 749-753		  
P-612  nuc  OEE2 genomic, EcoRI/KpnI fragment in pUC19 Mayfield et al. (1987)
  Proc. Natl. Acad. Sci. USA 84, 749-753		  
P-613
  pJN4		  nuc  CRY1 marker; RPS14 gene
  ca. 4 kb EcoRI fragment in pUC119		 Nelson et al. (1994)
   Mol. Cell. Biol. 14, 4011-4019		  
P-619  nuc  845 bp of cytochrome c6 5' UTR, in BSKS-
  sequence sufficient to drive copper-dependent expression		 Hill et al. (1991)
   J. Biol. Chem. 266, 15060-15067		  
P-620  nuc  120 bp of cytochrome c6 5' UTR in BSKS-
  sequence sufficient to drive copper-dependent expression		 Hill et al. (1991)
   J. Biol. Chem. 266, 15060-15067		  
P-628  nuc  NIC7 genomic, pnic7.9 Ferris (1995)
   Genetics 141, 543-549		  
P-640  nuc  Wild-type genomic NIT1 sequence
  marked with small insertion in intron 9;
   vector is a deletion derivative of pBR322		    
P-641  nuc  Wild-type genomic NIT1 clone, minus the promoter
  and a portion of the first exon,
  marked with a small insertion in intron 9;
  derived from P-640		    
P-642  nuc  Wild-type genomic NIT1 sequence, minus the
  four terminal 3' exons and the 3' untranslated region,
  and marked with a small insertion in intron 9
  insert derived from P-640 but in BSKS-		    
P-655  cp  E. coli aadA gene flanked by rps7 5' and rbcL 3';
  in Fargo et al. (1998) this was incorrectly
  listed as P-665		 Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-657  cp  E. coli aadA flanked by rps4 5' and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-663  cp  E. coli aadA flanked by atpB 5' (with S-D sequence eliminated) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-667  cp  Bam-HindIII (4.8 kb), 16S rRNA through 5' part of 23S rRNA
  contains spectinomycin resistance mutation spr-u-1-6-2 in 3' end of 16S rRNA
  same insert as P-228 but in Xl1 blue host strain		    
P-668  cp  psbA exon 5 to 23S rRNA 3', with DCMU4 and er-u-1a mutations  
P-671  cp  E. coli aadA flanked by rps4 5' (with S-D sequence eliminated) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-681  nuc   nuc lear aadA cassette; aadA (E. coli) flanked by RBCS2 5' and 3' Cerutti et al. (1997)
   Genetics 145, 97-110.		  
P-697  nuc  ble (phleomycin resistance gene from S. hindustanus) flanked by RBCS2 5' and 3' Stevens et al. (1996)
   Mol. Gen. Genet. 251, 23-30.		  
P-738  cp  E. coli aadA flanked by atpE 5' and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-739  cp  E. coli aadA flanked by atpE 5' (with S-D sequence eliminated) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-740  cp  E. coli aadA flanked by atpB5' (with canonical S-D sequence) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-741  cp  E. coli aadA flanked by rps4 5' (with canonical S-D sequence) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-742  cp  E. coli uidA (GUS) flanked by atpB 5' (with canonical S-D sequence) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-743  cp  E. coli uidA (GUS) flanked by rps4 5' (with canonical S-D sequence) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-744  cp  E. coli uidA (GUS) flanked by atpB 5' and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-745  cp  E. coli uidA (GUS) flanked by rps4 5' and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-746  cp  E. coli uidA (GUS) flanked by atpB 5' (with putative S-D sequence eliminated) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-747  cp  E. coli uidA (GUS) flanked by rps4 5' (with putative S-D sequence eliminated) and rbcL 3' Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-748  cp  Gene VII 5' UTR/ aadA coding, no S-D Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-749  cp  Gene VII 5' UTR/ aadA coding, canonical S-D Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-750  cp  Gene VII 5' UTR/ GUS coding, no S-D Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-751  cp  Gene VII 5' UTR/ GUS coding, canonical S-D Fargo et al. (1998)
   Mol. Gen. Genet. 257, 271-282 		 
P-762  cp  E. coli uidA flanked by petD 5' and rbcL 3'
   = David Stern's pDG2		    
P-763  cp  E. coli aadA flanked by petD 5' and rbcL 3'
   = David Stern's pDAAD		    

 

 

plasmid genome description reference more info
pSP124S  nuc  ble construct in which introns were inserted
  into the RBCS2 5' region to improve expression		 Lumbreras et al. (1998)
   Plant J. 14, 441-447		  
pSK.KmR  cp aphA-6 selectable marker flanked by psbA 5'and rbcL 3' Bateman and Purton (2000)
   Mol. Gen. Genet. 263, 404-410		  
pRbcRL(Hsp196)  nuc  synthetic luciferase reporter gene,
  controlled by HSP70A/RBCS2		 Fuhrmann et al. (2004)
   Plant Mol. Biol. 55, 869-881		 pdf file
pKScGFP  nuc  synthetic GFP reporter
  optimized for nuclear codon bias
  fused to ble, under control of RBCS2		 Fuhrmann et al. (1999)
   Plant J. 19, 353-361		 pdf file
p3xHA  nuc  Plasmid for epitope tagging
  contains 3 copies of the 9-amino acid
   influenza hemagglutinin epitope		 Silflow et al. (2001)
   J. Cell Biol. 153, 63-74		 tif file
pGenD  nuc  PSAD gene, from which coding region can be
   removed and replaced with a
   gene of interest under control
   of the PSAD flanking sequences		 Fischer and Rochaix (2001)
   Mol. Gen.Genet. 265, 888-894		  
pGenD-Ble  nuc  pGenD with ble insert Fischer and Rochaix (2001)
   Mol. Gen.Genet. 265, 888-894		  
pRbcBRL(Hsp196)  nuc  synthetic luciferase reporter gene,
  controlled by HSP70A/RBCS2		 Fuhrmann et al. (2004)
   Plant Mol. Biol. 55, 869-881		  
pRSET-PF16  nuc  6x-His-tagged PF16 fusion Smith and Lefebvre (1996)
   J. Cell Biol. 132, 359-370		  
pB6D2  nuc  genomic clone from PF16 gene
  capable of rescuing the pf16 mutant by transformation		 Smith and Lefebvre (1996)
   J. Cell Biol. 132, 359-370		  
pB1A10  nuc  probe for PF20 gene Smith and Lefebvre (1997)
   Mol. Biol. Cell 8, 455-467		  
pPF20  nuc  PF20 Smith and Lefebvre (1997)
   Mol. Biol. Cell 8, 455-467		  
pPF15  nuc  genomic clone of the PF15 gene Dymek et al. (2004)
   Eukaryotic Cell 3, 870-879		  
pC1a-86  nuc    Wargo et al. (2005)
  J. Cell Sci. 118, 4655-4665		  
pC1a-34  nuc    Wargo et al. (2005)
  J. Cell Sci. 118, 4655-4665		  
pC1a-32  nuc    Wargo et al. (2005)
  J. Cell Sci. 118, 4655-4665		  
pC1a-18  nuc    Wargo et al. (2005)
  J. Cell Sci. 118, 4655-4665		  
pEK RS3  nuc       
pCrGFP  nuc  synthetic GFP reporter
  optimized for nuclear codon bias
 		 Fuhrmann et al. (1999)
   Plant J. 19, 353-361		 map; cloning site
Maa7/XIR
(NE-537)		  nuc  construct for post-transcriptional gene silencing,
  based on tandem inverted repeat sequences from MAA7 		Rohr et al. (2004)
   Plant J. 40, 611-621		  
p5'FEA1-ARS2  nuc  ARS2 reporter
  under control of the iron-responsive FEA1 promoter		 Allen et al. (2007)
  Eukaryotic Cell 6, 1841-1852		  
p5'FOX1-ARS2  nuc  ARS2 reporter
  under control of the iron-responsive FOX1 promoter		 Allen et al. (2007)
  Eukaryotic Cell 6, 1841-1852		  
p5'FTR1-ARS2  nuc  ARS2 reporter
  under control of the iron-responsive FTR1 promoter		 Allen et al. (2007)
  Eukaryotic Cell 6, 1841-1852		  
pSI103  nuc  aphVIII (paromomycin resistance)
  with HSP70A/RBCS2 promoter		 Sizova et al. (2001)
   Gene 277, 221-229		 sequence [Word doc]
pJD54  nuc  ARS gene, with no promoter de Hostos et al. (1989)
   Mol. Gen. Genet. 218, 229-239		  
pJD100  nuc  arylsulfatase reporter with beta-tubulin promoter Davies et al. (1992)
   Nucl. Acids Res. 20, 2959-2965		  
pML9.7  nuc  genomic clone of UNI2 gene Piasecki et al. (2008)
   Mol Biol Cell. 19, 262-273		  
pML9.7-3xHA  nuc  UNI2 gene tagged with 3xHA
  (3 copies of the 9-amino acid influenza hemagglutinin epitope)		 Silflow etal. (2001)
   J. Cell Biol. 153, 63-74
Piasecki et al. (2008)
   Mol. Biol. Cell 19, 262-273		  
pHsp70A/
  RBCS2-Chlamy		  nuc  loxP, codon-adapted Renilla luciferase
  under control of HSP70A/RBCS2 promoter with
  RBCS2 intron 1 and 3' UTR		 Heitzer and Zschoernig (2007)
   Biotechniques 43, 324		 pdf file
pHsp70A/
  RBCS2-cgLuc		  nuc  codon-adapted Gaussia princes luciferase
  under control of HSP70A/RBCS2 promoter		 Fuhrmann et al. (2004)
   Plant Mol. Biol. 55, 869-881
Heitzer and Zschoernig (2007)
   Biotechniques 43, 324		 pdf file
pHsp70A/
  RBCS2-cEpo 		 nuc  loxP, HSP70A/RBCS2 promoter, codon-adapted
  human erythropoietin with ARS2 leader and
  RBCS2 introns, His6 tag, RBCS2 3' UTR		 Fuhrmann et al. (2004)
   Plant Mol. Biol. 55, 869-881
Heitzer and Zschoernig (2007)
   Biotechniques 43, 324		 pdf file
pAES14  nuc  ARS2 promoter, codon-adapted crluc
  Renilla luciferase with RBCS2 introns, Hs6 tag;
  kanamycin resistant		 Fuhrmann et al. (2004)
   Plant Mol. Biol. 55, 869-881		 pdf file
pUC18-Arg7
-lox-B		  nuc  ARG7.8 genomic sequence inserted into pUCBM20,
  followed by loxP site		 Heitzer and Zschoernig (2007)
   Biotechniques 43, 324		 pdf file
pKS-aphVIII-lox  nuc  expression vector containing loxP, beta-tubulin promoter,
  aphVIII, and 3' UTR from chlamyopsin-1		 Heitzer and Zschoernig (2007)
   Biotechniques 43, 324		 pdf file
pKS-aph7``-lox  nuc  expression vector containing loxP, beta-tubulin
  promoter, aph7 with intron 1 from RBCS2,
  and 3' UTR from chlamyopsin-1		 Heitzer and Zschoernig (2007)
   Biotechniques 43, 324		 pdf file
pBACmn   plasmid used in preparing the BAC library   sequence [text file]
pChlamiRNA2 nuc artificial mRNAi construct Molnar et al. (2009), Plant Journal in press, PMID: 19054357 web link
pChlamiRNA3 nuc artificial mRNAi construct Molnar et al. (2009), Plant Journal in press, PMID: 19054357 web link
pChlamiRNA3int nuc artificial mRNAi construct Molnar et al. (2009), Plant Journal in press, PMID: 19054357 web link

Return to Chlamydomonas Center, strain information

Return to Chlamydomonas Center home page